Since 1 April 2011, the 90-day trial period is available to all employers, not just those with less than 20 employees at the time of hiring.
You may not raise a personal grievance in respect of a dismissal that occurs during the 90-day period, providing that the trial period has been correctly entered into.
You must agree to the trial period with your employer prior to starting work. The 90-day trial period is only open to 'new' employees. If you have commenced work prior to signing the agreement, then the 90-day trial period will not apply, and your employer may not rely on that period to dismiss you.
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